Obligatory base module 2 The Learning outcomes
By the end of the course students will be able to:
1) write scientific report
2) analyse the results of the experiment
Students will become familier with the following techniques:
1) Chemical titration
2) Western blot
3) PCR
4) Gel electrophoresis
5) DNA extraction
6) Plasmid purification
7) restriction analysis
8) preparation and negative staining of human oral microbiome to explore the morphology of different microbes inhabiting oral cavity;
9) preparation and simple staining of a pure culture of one bacterial strain;
10) determination of susceptibility/resistance of different bacteria to lyzosyme;
11) determination of the ability to use citrate and urea as carbon source by different bacteria;
12) Sub-culturing attached cell lines requiring trypsin;
13) Thawing frozen cells;
14)Fixing attached cells in paraformaldehyde
15) Investigation of random quantity distribution law using AC grid voltage
16) Determining the power and efficiency of a solar cell
Students will be able to operate simple light microscope, construct LEGO robot.
Brief description of content
The lab course will introduce laboratory methods and techniques from the fields of physics, chemistry, genetics, micro-, molecular and cell biology, genetechnology, bioengineering and robotics.. Students will get both theoretical knowledge and practical experience in different methods like chemical titration, Western blot, PCR, Gel elecrtophoresis, DNA extraction, use microscope to determine bacteria, get hands-on expirience in LEGO robot construction. Furthermore, students will learn how to write scientific report, analyse the obtained results, troubleshoot. Students will complete practical assignments and tests, write and submit reports.
Students will improve their skills in:
1) writing scientific report
2) analysing the results of the experiment
Students will learn to operate simple light microscope, construct LEGO robot.